Monday, November 4, 2019
Creative Media Essay Example | Topics and Well Written Essays - 2250 words
Creative Media - Essay Example These building blocks include the sounds and images. The value of aesthetics in animation cannot be quantified. It may appear subtle. However, aesthetics in animation plays a vital role. In other fields like cinemas, the content within a storyline as well as the storyline itself play the aesthetic role. By use of aesthetics, a story may be delivered as intended, or it may deliver the contradictory message. The thrill in collaboration of images and pixels of objects surpasses the verbal grasp. For this reason, animation may appear to be imaginary or real (Kreowski & Ehrig, 2005). In order to deliver the best aesthetics in animation, one needs to consider the coherence of the artificial models. Poor aesthetics, on the other hand, may be able to result in delivery of the wrong message. This could make a film to appear disengaging or even unprofessional. When making animated films, attention to aesthetics is inevitable. Proper aesthetics helps the audience to build their trust in the product. Consequently, they may forget that they are watching a film. This helps in impacting the intended emotions in the audience (Pikkov, & NaÃËripea, 2010). In animation, style may be referred to as the preferences of the filmmaker in the choice of aesthetics. A filmmakerââ¬â¢s style may be determined by the kinds or models of worlds that he chooses to lean on. The choice of style depends on the consideration of its purity and authenticity. In addition, they may choose to exploit their prowess, experience and knowledge. Often, the filmmakers may not be quite aware of their choice of style. These filmmakers perform their chores so faithfully and naturally (Carroll, 2001). Style generates itself through a combination of ideals of the filmmaker. In essence, style emanates through the product as compared to the inputs. Sometimes, style may be misinterpreted as the superficial change of the appearance of an
Friday, November 1, 2019
Construction & Diagnostic of Recombinant DNA Plasmid Research Paper
Construction & Diagnostic of Recombinant DNA Plasmid - Research Paper Example The experiment established that the competency of cells increased greatly.Ã In the next experiment, running PCR provided me with an amplified His3 gene which is used in subsequent steps to transform haploid yeast. Transformation occurred through homologous recombination. The experiment proved that specific integration does occur although cases of non-specific integration are rampant. In the next experiment, I constructed a sub-clone of the HIS3 gene and inserted it into a plasmid pSP72 making a recombinant plasmid which I used to transform bacterial cells. In order to determine whether the integration was successful, I extracted the plasmid to analyze if the inserted gene was present. However, I established that integration had not been successful. Introduction One of the laboratory techniques that help in understanding the basics of knocking out genes is the standardized procedure of replacing the ADE2 gene responsible for adenine biosynthesis with HIS3 that is responsible for on e of the steps in histidine amino acids. The procedure involves production of a hybrid polymerase chain reaction (PCR) as the first step. The hybrid product constitute of the vector DNA template and the primers of choice. Running of the PCR major steps produces the hybrid product. Agarose gel electrophoresis helps in determining whether PCR amplification occurred. The next step involves the transformation of yeast cells with the hybrid PCR product. The last step involves analysis of r4esults and morphology of transformed yeast cells. Other experiments involve transforming competent Escherichia coli cells with the use of a plasmid as a vector. This transformation follows the recombinant DNA technology protocol. The general procedure starts with digestion of plasmid DNA and template DNA of interest with restriction enzymes to generate DNA fragments with sticky ends. The second step involves ligation of the DNA fragments using DNA ligase, forming a recombinant plasmid. The next step in volves insertion of the recombinant plasmid into the competent bacterial cells. The final step involves plating on appropriate media and selection of transformed cells. In addition, performing a backward procedure of isolating the plasmid from the transformed cells verifies insertion at the right locus. Laboratory 1: Transformation of Competent Bacteria Objective: Introduction of Plasmid DNA into E. coli cells and determination of transformation efficiency Materials and Reagents: Plasmid DNA Gene of interest SOC media LB-amp media Procedure: The protocol preferred was the High-Efficiency Transformation Protocol. However, a variation occurred with 2Ã µl of plasmid DNA. The high efficiency transformation protocol requires thawing of competent cells in ice for about ten minutes. The next step involves transfer of 50Ã µl of the cells to a transformation tube using a micropipette. Adding of 2Ã µl of plasmid DNA into the tube followed. The next step involved placing the mixture on ice for 20 minutes. Next, exposure of cells to heat shock at 42?C occurs, a process lasting 35 seconds. Following this was adding the right amount of SOC media to the cells. After this, incubation at 37C? for 40 minutes and subsequent vigorous shaking followed. Plating of the cells in LB-amp media and overnight incubation at 37?C was the last step. In the first experiment, plating of the concentrated cell mixture without dilution occurred. In subsequent trials, there was dilution of cell solution at different dilution ratios.
Wednesday, October 30, 2019
Quick Loan Business Essay Example | Topics and Well Written Essays - 750 words
Quick Loan Business - Essay Example The requirements for taking out a payday loan are very easy. Therefore, it is very likely for any individual to take out the payday loans so long as one has a bank account, some form of income such as benefits like social security as well as a legal form of identification. This kind of loan assists people in coping with the expenses that come in between their paycheck (Bartlett, 2009. A payday loan is very important both to the borrower and to the lender. This is because as long as the borrower has a bank account, the loan can be available. Even if the borrower had been declared bankrupt in the past, or had been having a bad credit history, the individual is in a position to acquire a payday loan. This is in contrast with other lending companies which cannot give a loan to individuals with bad credit history as well as to people who are bankrupt. This means that the borrower needs not to worry about accessing payday loan. The author says that payday loans are taken very quickly. This is because of the low requirements that are needed to acquire the loan as well as their design for the short time. The author says that the payday loan can be available within 24 hours after application, even if some companies make the funds available in just a couple of hours. This means that the choices are great if the borrower needs the money quickly. The money can be available instantly if the borrower has the unexpected expense (Francis, 2009). Payday lenders offer small amounts of money which is in contrast with other lending companies whereby they put an upper limit on how much an individual can borrow. As the author puts it, the limit means that the borrower cannot borrow more than what an individual can be able to pay. The author explains that the borrower cannot get into more debts as part of the procedure Lawrence & Elliehausen, 2008).
Monday, October 28, 2019
Liquids and Solids Essay Example for Free
Liquids and Solids Essay The purpose of this experiment is to examine the properties of several gasses, which were the products of a reaction, and examine the way the gasses react under certain conditions. These conditions, such as introducing a flame to the gas as well as oxygen and CO2, caused other reactions to occur. Experiment Before I began the experiment I gathered all my supplies from the given list in the lab manual. From there I began the actual experiment and diluted the HCL placed in a test tube and then placed the ZN in the tube as well. I placed the stopper and gas delivery plastic system on the test tube and arranged the tube in the well plate. I then took the test tube filled with water inverted it and placed on top of the gas delivery system, I had the well plate in a large bowl in case of flow over. I then removed and placed my thumb over the opening so gas wouldnââ¬â¢t escape I lit a match and held it approx. 1 cm away and allowed a small drop from the pipet onto the flame to observe the reaction. From here I took my pipet bulb and marked it in 3 equal parts with a marker. I filled the pipet with water and set it into the gas generation tube. When it was about 2/3 full of gas I removed it and set it facing down into the well holder as per the directions. I then cleaned up the other test tube disposed of the acid and washed the test tube and rubber stopper with water thoroughly. I then began the oxygen portion of the experiment where I placed some Mn into a test tube. I added some hydrogen peroxide and filled to nearly the top. I placed the rubber stopper with gas delivery system and put into the well plate in a straight position. I filled the pipet with water and once again inverted it and placed in on top of the gas delivery system. I then watched as it became displaced with oxygen and removed it placed my thumb over it so it wouldnââ¬â¢t escape.
Saturday, October 26, 2019
Wilhelm Reich Essay -- Biography Bio
Wilhelm Reich was born in the Austro-Hungarian Empire on March 24th, 1897. His parents were farmers, and at a young age Reich developed a fascination with the life processes of plants and animals. His formal education at this time was provided by a private tutor. When Reich was 14, he discovered his mother was having an affair with his tutor. After Reich reported this to his father, his mother committed suicide. Atwood and Stolorow (1977) have speculated that this tragedy may have contributed to Reich's most significant theories. After the death of his father three years later, Reich took over the family farm until it was destroyed by the Russians in 1915. At that time he joined the Austrian Army. Upon returning from the war, Reich traveled to Vienna to study medicine at the University. In 1922 he received his medical degree. That same year, Freud organized the Psychoanalytic-Polyclinic and appointed Reich the first assistant physician. Over the next few years, Reich was appointed to the teaching staff of the Psycholoanalytic Institute. He married and had two daughters. During this time he became increasingly convinced of the absolute significance of sexuality in the lives of individuals and society. He believed that social institutions, the family in particular, forced their members to repress natural sexual energy. Consequently, this energy builds up inside these repressed individuals. With no socially acceptable outlet, the build-up becomes intolerable and manifests itself in neuroses. Reich believed the way this energy was intended to be released was through orgasm. However, he contended that orgasm alone was not sufficient in and of itself to release all the excess energy stored by an individual. A person must be ... ...colleagues were also renting and selling orgone accumulators to physicians who were prescribing them to patients for therapeutic purposes. It was this activity which led to an injuction against Reich being filed by the Food and Drug Administration in 1954. The FDA charged that orgone energy accumulators were fraudulent medical devices, that orgone energy did not exist, and that all literature concerning orgone energy should be burned. Additionally, they prohibited Reich from transporting the accumulators across state lines. In 1955, official contempt charges were placed against Reich and Dr. Michael Silvert after Silvert continued to distribute the accumulators out of state. Both were tried in a criminal court, found guilty, and sentenced to imprisonment. Reich never finished his prison term; he died on November 3, 1957 in a penitentiary in Lewisburg, Pennsylvania. Wilhelm Reich Essay -- Biography Bio Wilhelm Reich was born in the Austro-Hungarian Empire on March 24th, 1897. His parents were farmers, and at a young age Reich developed a fascination with the life processes of plants and animals. His formal education at this time was provided by a private tutor. When Reich was 14, he discovered his mother was having an affair with his tutor. After Reich reported this to his father, his mother committed suicide. Atwood and Stolorow (1977) have speculated that this tragedy may have contributed to Reich's most significant theories. After the death of his father three years later, Reich took over the family farm until it was destroyed by the Russians in 1915. At that time he joined the Austrian Army. Upon returning from the war, Reich traveled to Vienna to study medicine at the University. In 1922 he received his medical degree. That same year, Freud organized the Psychoanalytic-Polyclinic and appointed Reich the first assistant physician. Over the next few years, Reich was appointed to the teaching staff of the Psycholoanalytic Institute. He married and had two daughters. During this time he became increasingly convinced of the absolute significance of sexuality in the lives of individuals and society. He believed that social institutions, the family in particular, forced their members to repress natural sexual energy. Consequently, this energy builds up inside these repressed individuals. With no socially acceptable outlet, the build-up becomes intolerable and manifests itself in neuroses. Reich believed the way this energy was intended to be released was through orgasm. However, he contended that orgasm alone was not sufficient in and of itself to release all the excess energy stored by an individual. A person must be ... ...colleagues were also renting and selling orgone accumulators to physicians who were prescribing them to patients for therapeutic purposes. It was this activity which led to an injuction against Reich being filed by the Food and Drug Administration in 1954. The FDA charged that orgone energy accumulators were fraudulent medical devices, that orgone energy did not exist, and that all literature concerning orgone energy should be burned. Additionally, they prohibited Reich from transporting the accumulators across state lines. In 1955, official contempt charges were placed against Reich and Dr. Michael Silvert after Silvert continued to distribute the accumulators out of state. Both were tried in a criminal court, found guilty, and sentenced to imprisonment. Reich never finished his prison term; he died on November 3, 1957 in a penitentiary in Lewisburg, Pennsylvania.
Thursday, October 24, 2019
The Controversial Issue of Affirmative Action :: Affirmative Action Racism Racist Essays
The Controversial Issue of Affirmative Action Considering the subject of affirmative action the following questions frequently are raised: Is there a clear understanding of affirmative action roles/goals? What are the pros/cons of these programs? What are the "loop holes" in the system? Does seniority play a role in affirmative action? Addressing these key questions may help us all in our daily routine, as administrators and/or potential administrator in the public/private sector. Affirmative action programs throughout the United States have long been a controversial issue particularly concerning employment practices (public/private) and university student and/or staff recruitment. Most public agencies have some type of instituted affirmative action program. According to Cheryl Perry-League, Director of Equal Opportunity of the Port of Oakland, every business operating on Port of Oakland owned land must have a standing affirmative action program on record and businesses bidding to do work for the Port of Oakland must have an acc eptably diverse workforce. BACKGROUND To understand the role and/or goals of affirmative actions programs we should define what the broad definition of what affirmative action is and what caused its development. The phase "affirmative action" was used in a racial discrimination context. Executive Order No. 10,925 issued by President John F. Kennedy in 1961. The order indicated that federal contractors should take affirmative action to ensure job applicants and employees are treated "without regard to their race, creed, or national origin." A person could define this statement as an order to imply equal access and nothing else. Subsequently, Executive Order 11246 issued by President Johnson in September 1965, "mandated affirmative action goals for all federally funded programs and moved monitoring and enforcement of affirmative action programs out of the White House and into the Labor Department." Affirmative action "refers to various efforts to deliberately take race, sex, and natio nal origins into account to remedy past and current effects of discrimination. Its primary goal is to ensure that women and minorities are widely represented in all occupations and at all organizational levels" (Tompkins, 1995, p.161). Another definition of affirmative action according to Barbara Bergmann is "planning and acting to end the absence of certain kinds of people-those who belong to groups that have been subordinated or left out-from certain jobs and schools" (1997 p.7). Tracing the history of affirmative action, laws against racial discrimination have proved inadequate for workplace integration because they often provide remedies only after the fact.
Wednesday, October 23, 2019
Norton Lily
PHASE 1 OF THE TURNAROUND : Building The Foundation Jim Burton set about analyzing the business with an emphasis on quick wins that would contribute to developing the long-term ââ¬Å"executionâ⬠foundation. * He was dealing with seasoned industry veterans, each with deaply held beliefs and paradigms about the business ââ¬â how it had been and should be run. In an effort to build confidence and gain acceptance among his senior peers ( it had to be an incremental approach) * With encouragement and support of the owner, burton first formed an executive committee of nine, including the five business unit.The objective in forming executives committee was to begin decentralizing the decision making process away from the two owner. ( The owners not actively participate in committee sessions, but would instead attend summary reviews, at the conclusion of each meeting, to provide counsel and advice ) * He was to transform the company and build the sustainable growth platform the own er wanted Identifying and Understanding Value Creating Processes Burton focused on its execution and on helping Norton Lillyââ¬â¢s executives understand the key processes that enabled good strategy execution and value creation. Burton first launced process mapping in the linier group, which operated in eight different US offices * At the time, the liner division offered a fragmented mix of services across its eight location, with each location performing some activities more effectively than other location and all location failing to provide adequate service in some regard * By late 2007, mapping the key processes involved in delivering each type of service had helped management and employee understanding of processes led to improved process standardization, end administrative procedures that duplicated work, improved costumer satisfaction, and penalties * By the end of 2007, Burton had assigned responbility for achieving KPI goals to individual managers and supervisor overseeing specific value creating process within the liner division Implementing a Balanced Scorecard Performance Measurement System Accompanying the KPIââ¬â¢s rollout was a balanced scorecard, wich further focused managerial employees attention on the performace of value creating process. Metrics included in the balance scorecard system used at Norton Lilly included process KPIââ¬â¢s. costumer satisfaction and financial performance * In late 2007 Norton Lillyââ¬â¢s balance scorecard system was expanded to included a ââ¬Å" dashboardâ⬠of performance indicators that could provide overview of business unit level * The dashboard contained : * Revenue compared to budget * Expense groupings compared to budget * Capital expenditures compared to budget Top 10 Costumer profitability * By the end of 2007 Norton Lilly business unit leaders were holding monthlymeetings to review KPI status and propose corrective action to resolve differences between expectations and actual performance R esource Allocation Policies Other foundation building actions undertaken in 2007 included in the initation of various policy changes. One such policy change involved the companyââ¬â¢s capital outlay policy, which was the first new policy established by the executive committee. * The capital outlay policy came about after Burton discovered that the company had variously launched into a number of ventures without much success. The policy called for a nine step due diligence process, that nine steps were as follows: 1. Description of the opportunity 2. Description of how the opportunity fit with Norton Lillyââ¬â¢s objectives 3. Assessment of the competitive threats 4. Assessment of the competitive landscape 5. How success whouldbe ensured 6. Proposed exit strategy 7. Business case 8. Financila pro forma 9. List of major assumtions and risk * The capital outlay policy would ensure that decision to entef into any business venture would be fully vetted, based on fact, not opinions B uilding Managerial Talent Throught out 2007 Norton Lilly focused on another important foundation building element management development. * Burton introduced a leadership evelopment program that matched a personââ¬â¢s profile to a job, the program was based on the fundamental premise that managerial employees could be classified into one of four basic profil; stategist, project director, networker or external qualifier * After being tested and profiled, the 20 highest-rangking employees at Norton lilly entered into workshops directed at helping members of the management team interpreteach profile and respect and deal effectively with differing profiles PHASE II OF THE TURNAROUND REINFORCING THE FOUNDATION The effort undertaken during Phase 1 of Norton Lillyââ¬â¢s turnaround had produced a profitable fiscal 2007, which was the companyââ¬â¢s first profitable year since 2003. Improving Information Used for Decision Making Dashboard were also expanded to all of Norton Lillyâ⠬â¢s business units.Linerm Ship services, and Overseas. As well as the addiction of Financial measures such as cash flow and accounts receivable KPIââ¬â¢s Changing Financial Performance Expectations Burton initiated another policy change in 2008, although less a stated policy than a mindset. * The concept of value creation was introduced at an executive committee meeting in late 2007 and became the basis for all 2008 budgeting * The concept of earning a fair rate of return based on assumed risk didnââ¬â¢t exist at Norton Lilly, so as apart of the 2008 budgeting exercise each business unit leader was given a margin target A NEW APPROACH TO FORCASTING FINANCIAL PERFORMANCE Provide continual refreshing of the assumptions underlying the forecast * Provide the company a continual look at its next 12 months, regardless of how many months were left in the calendar year The move to rolling forecast helpd managers improve forecasting accuracy to the extent that the company missed its financial projections. TAHAP 1 DARI TURNAROUN : Membangun Pondasi Jim Burton berurusan dengan veteran industri berpengalaman, masing-masing dengan keyakinan dipegang dan paradigma tentang bisnis ââ¬â bagaimana hal itu telah dan harus dijalankan. Dalam upaya untuk membangun kepercayaan dan mendapatkan penerimaan di antara rekan seniornya (itu harus pendekatan inkremental) à à à à à à Dengan dorongan dan dukungan dari owner, Burton pertama membentuk executive committee of nine, termasuk lima bisnis unit. Tujuan dalam membentuk executive committee adalah untuk mulai desentralisasi proses pengambilan keputusan dari kedua owner . owner tidak aktif berpartisipasi dalam sesi komite, melainkan akan menghadiri summary review, pada akhir / kesimpulan setiap pertemuan, untuk memberikan nasihat dan saran) à à à à à à Jim Burton mengubah perusahaan dan membangun sustainable growth platform Mengidentifikasi dan Memahami Value Creating Processes Burton berfokus pada p elaksanaannya dan membantu Norton Lillyââ¬â¢s executives memahami key process yang memungkinkan strategi eksekusi yang baik dan penciptaan nilai. Burton pertama meluncurkan proses pemetaan pada kelompok kapal , yang beroperasi di delapan kantor yang berbeda o à à à Pada saat itu, divisi kapal menawarkan berbagai pelayanan terfragmentasi di delapan lokasi, dengan masing-masing lokasi melakukan beberapa kegiatan yang lebih efektif daripada lokasi lain. an lokasi semua gagal untuk memberikan layanan yang memadai dalam hal tertentu à à à à à à Pada akhir 2007, pemetaan key process yang terlibat dalam memberikan setiap jenis layanan telah membantu manajemen dan karyawan memahami proses untuk memperbaiki proses standarisasi, mengakhiri prosedur administrative yang tidak efektif , meningkatkan kepuasan pelanggan. Pada akhir tahun 2007, Burton diberi tanggung jawab untuk mencapai tujuan KPI untuk manajer individu dan supervisor mengawasi spesicific value creating proce ss dalam divisi kapal Menerapkan Kinerja Balance Scorecard Sistem Pengukuran Seimbang Sistem balanced scorecard fokus ke perhatian manajerial karyawan pada performance value creating process.Metrik yang termasuk dalam sistem balance scorecard digunakan di Norton Lilly termasuk proses KPI kepuasan pelanggan dan kinerja keuangan à à à à à à Pada akhir tahun 2007 sistem balance scorecard Norton Lilly diperluas untuk memasukkan ââ¬Å"dashboardâ⬠indikator kinerja yang dapat memberikan gambaran tingkat unit bisnis o à à à à à à à Dashboard berisi: Pendapatan dibandingkan dengan anggaran Pengelompokan Expence dibandingkan dengan anggaran à à à à à à Pengeluaran modal dibandingkan dengan anggaran à à à à à à Top 10 Costumer profitabilitas Pada akhir tahun 2007 para pemimpin unit bisnis Lilly Norton memegang monthlymeetings untuk meninjau status KPI dan mengusulkan tindakan korektif untuk mengatasi perbedaan antara harapan dan kine rja aktual Alokasi sumber daya Kebijakan Tindakan membangun pondasi lainnya dilakukan pada tahun 2007 termasuk dalam inisiasi perubahan berbagai kebijakan.Salah satu perubahan kebijakan tersebut melibatkan kebijakan pengeluaran modal perusahaan, yang merupakan kebijakan baru pertama kali didirikan oleh komite eksekutif. Kebijakan pengeluaran modal muncul setelah Burton menemukan bahwa perusahaan telah meluncurkan beberapa usaha tanpa banyak keberhasilan. Kebijakan menyerukan sembilan langkah due diligence process karena sembilan langkah tersebut adalah sebagai berikut: 1. Deskripsi kesempatan 2. Deskripsi bagaimana kesempatan fit dengan Norton Lilly objectives 3. Penilaian terhadap ancaman kompetitif 4. Penilaian terhadap kompetitif landscape 5. Bagaimana keberhasilan bias dipastikan 6. Usulan exit strategy 7. Kasus bisnis 8. Financial pro forma 9. Daftar asumsi utama dan resikoKebijakan pengeluaran modal akan memastikan bahwa keputusan untuk masuk ke setiap usaha bisnis akan sepenu hnya diperiksa, berdasarkan fakta, bukan pendapat Membangun Bakat Manajerial Norton Lilly terfokus pada perkembangan foundation building elemen management development yang lain à à à à à à Burton memperkenalkan program pengembangan kepemimpinan menyesuaikan profil seseorang sesuai dengan pekerjaannya , program ini didasarkan pada premis dasar bahwa karyawan manajerial dapat diklasifikasikan ke dalam salah satu dari empat profil dasar; stategist, direktur proyek, networker atau eksternal kualifikasi à à à à à à Setelah diuji dan diprofilkan, 20-rangking karyawan yang tertinggi di Norton lilly engadakan workshop diarahkan untuk membantu anggota tim manajemen saling memahami, menghormati dan menangani secara efektif. FASE II DARI TURNAROUND: Memperkuat Pondasi Upaya yang dilakukan selama fase 1 dari turnaround Norton Lilly telah menghasilkan fiskal tahun 2007 yang menguntungkan, yang mana ini menjadi tahun pertama yang menguntungkan perusahaan sejak tahun 2003 . Meningkatkan Informasi Digunakan untuk Pengambilan Keputusan Dashboard juga diperluas ke semua unit bisnis Norton Lilly. Serta penambahan ukuran finansial seperti arus kas dan rekening yang piutang KPI Mengubah Harapan Kinerja Keuangan Burton memulai lagi perubahan kebijakan pada tahun 2008, walaupun kurang kebijakan dinyatakan dari pola pikir.Konsep penciptaan nilai diperkenalkan pada pertemuan komite eksekutif pada akhir 2007 dan menjadi dasar bagi semua penganggaran 2008 à à à à à à Konsep a fair rate of return berdasarkan risiko diasumsikan tidak ada di Norton Lilly, sehingga terpisah dari budgeting exercise 2008 masing-masing pimpinan unit bisnis diberi target marjin Pendekatan baru untuk peramalan kinerja keuangam Meng-udate atau refresh terus-menerus asumsi yang mendasari forcasting à à à à à à Memberikan perusahaan melihat terus-menerus pada 12 bulan berikutnya, terlepas dari berapa bulan yang tersisa di tahun kalender. Hal tersebut membantu manajer meningkatkan akurasi peramalan saat perusahaan membutuhkan proyeksi keuangan.
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